Biophysical research facilities at Centre of molecular structure

Tatsiana Charnavets, Olga Dzmitruk, Bohdan Schneider, Jan DohnŠlek

Institute of Biotechnology CAS, v.v.i., BIOCEV, PrýmyslovŠ 595, Vestec, 52542, Czech Republic

The biophysical research facility as a part of the Centre of molecular structure of the Institute of Biotechnology is a member of the Instruct-ERIC Research Infrastructure in the Czech Infrastructure for Integrative Structural Biology (CIISB) and also a member of the Molecular-Scale Biophysics Research Infrastructure (MOSBRI). The biophysical research facility provides shared resources of instruments and technologies for the determination of size, structure and stability of biomolecules, study of conformational changes and thermodynamics of temperature transitions and characterization of biomolecular interactions.

The following instruments and technologies are currently available: circular dichroism spectroscopy (Chirascan Plus CD spectrometer), spectrophotometry (Specord 50 Plus UV/Vis spectrophotometer), Fourier-transform infrared spectrometry (Vertex 70v spectrometer), fluorescence spectrometry (photoluminescence spectrometer FLS1000), differential scanning fluorescence (Prometheus NT.48), multiangle dynamic light scattering (Zetasizer Ultra), microplate-reader (Tecan) differential scanning calorimetry (Microcal VP-DSC), isothermal titration calorimetry (Microcal iTC200), microscale thermophoresis (Monolith NT.115 and NT.LabelFree), surface plasmon resonance (ProteOn XPR36) and bio-layer Interferometry (OCTET R8).

Users can apply for access to the biophysical instrumentation and expertise via CIISB (https://stigmator.ceitec.muni.cz/project_form/), Instruct (https://instruct-eric.eu/submit-proposal), MOSBRI (https://www.mosbri.eu/start-tna-proposal/).

The Centre of Molecular Structure is supported by: MEYS CR (LM2018127); project Czech Infrastructure for Integrative Structural Biology for Human Health (CZ.02.1.01/0.0/0.0/16_013/0001776) from the ERDF; UP CIISB (CZ.02.1.01/0.0/0.0/18_046/0015974), ELIBIO (CZ.02.1.01/0.0/0.0/15_003/0000447), and MOSBRI from EU Horizon 2020 (No. 101004806).