2Anton Paar GmbH, Anton-Paar-Strasse
20, Graz
Small angle X-ray scattering (SAXS) serves as one of the complementary methods of structural biology. SAXS allows both characterization and low resolution structural studies of macromolecules, with advantage of liquid samples. Several such complementary methods can be directly coupled with SAXS. Thanks to the high flux X-ray sources like MetalJet (Excillum), these applications are now possible not only at sunchrotrons, but also in-house. Here, in situ UV-Vis absorbtion spectroscopy and size exclusion liquid chromatography coupled with SAXS, as available in Centre of Molecular Structure, will be presented.
Centre of Molecular Structure is equipped with SAXSPoint 2.0 (Anton Paar) instrument with MetalJet C2+ X-ray source and Eiger 2M detector. Samples can be loaded to variety of the capillaries using an autosampler. The set of temperature controlled sample stages include low noise cell and capillary capable of in situ UV/Vis absorption spectrometry. Recently, a dedicated liquid chromatography system AktaGo (GE Healthcare) was introduced.
The Centre of Molecular Structure is supported by:
MEYS CR (LM2018127); project Czech Infrastructure for Integrative Structural
Biology for Human Health (CZ.02.1.01/0.0/0.0/16_013/0001776) from the ERDF; UP
CIISB (CZ.02.1.01/0.0/0.0/18_046/0015974), a
ELIBIO (CZ.02.1.01/0.0/0.0/15_003/0000447).