Crystallization studies of recently prepared haloalkan dehalogenas DgaA of Glacieocola agarylitica NO₂

Ivana Berkova¹, Tatyana Prudnikova^1,2, Michal Kuty^1,2, Radka Chaloupkova³, Jiri Damborsky³, Ivana Kuta Smatanova^1,2

¹University of South Bohemia in Ceske Budejovice, Faculty of Science, Branisovska 1760, 370 05 Ceske Budejovice

²Academy of Science of the Czech Republic, Center for Nanobiology and Structural Biology IMB, Zamek 136, 373 33 Nove Hrady

³1Loschmidt Laboratories, Department of Experimental Biology and Research Centre for Toxic Compounds in the Environment RECETOX, Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno, Czech Republic

The main goal is focused on crystalization study of newly prepared haloalkan dehalogenase DgaA from bacteria Glaciecola agarylitica NO2. The main target of this work is getting acquianted with methods of protein crystalization and usage of those methods for preparation of suitable DgaA protein crystals of that will be used for X-ray structural analysis. An essential requirement for using X-ray diffraction is to produce well ordered crystals without defects that are large enough to provide diffraction data after passing the X-ray beam. The crystallization experiment was performed using the sitting drop vapour diffusion techniques and crystallization screen Index HR2-144 (Hampton Research, USA). Results from difraction analysis of DgaA crystals will be starting point for further research focused on structure determination and and description of protein function.

Acknowledgement: The work was supported from GACR 17-24321S.