The Core facility X-ray diffraction and Bio-SAXS of the CEITEC-MU located in Brno was inaugurated in September 2013. Since then, the core facility (CF) offers access to the monocrystal X-ray diffractometers and Bio-SAXS Kratky camera system. The list of CF services includes collection of diffraction and scattering data, X-ray structure determination, Bio-SAXS data analysis, long-term cryogenic storage of crystals or hardware support for synchrotron trips.
Here, selected examples from users community illustrates the possibilities of SAXS data analysis on biological samples as ab initio shape reconstruction, quaternary structure model building of macromolecular complexes and studies of intrinsically disordered proteins. The complex formation of an intrinsically disordered protein and telomeric DNA was chosen as a case study not straightforward to analyse by other methods of structural analysis. Two-phase ab initio SAXS-based modeling was used to reconstruct the shape of telomeric repeat-binding factor with telomeric DNA duplex.
This work was supported by the CIISB research infrastructure project (LM2015043) funded by MEYS CR.