Crystallization of proteins is a multiparameter process that is very difficult predictable. Crystallization process is still the limiting step to obtain accurate 3D model of proteins by the means of X-ray (neutron) diffraction technique. The number of parameters affecting nucleation and growing of well-ordered crystals is too high and its prediction entails a degree of complexity comparable to the simulation of the protein-folding process.
There are several techniques can be used to drive system into the supersaturation region, but mainly three of them are used the most frequently: batch, vapor diffusion and liquid-liquid diffusion [1]. Using of different crystallization techniques should be considered when trying to improve crystal quality.
Crystallization procedures and parameters affecting crystal growth will be discussed during the lecture.
This research is supported by the GACR P207/12/0775.