Course of experimental techniques in structure analysis

some of the lectures will be common for colloquium Struktura 2013 and the course

 Foundations of diffraction methods

  Václav Petříček, Institute of Physics, AS CR                                                                                                               

                                                      Principles of diffraction methods, data reduction and corrections               

                                                      Symmetry and determination of space group                                                      

                                                     Structure refinement                                                                                                        

                                                     Twinning, modulation                                                                                                                                                        

Instrumental details – production and detection of X-rays, sample preparation

Karla Fejfarová, Institute of Macromolecular Chemistry, AS CR   

                                                      Sources of X-ray radiation

 Tereza Skálová, Institute of Macromolecular Chemistry, AS CR   

                                                      Detectors of X-rays used for  structure determination                                       

  Synchrotron sources

Pavlína Řezáčová, Institute of Organic Chemistry and Biochemistry, AS CR   

                                                      Experimental techniques for protein crystallography in USA                           

Tomáš Koval, Institute of Macromolecular Chemistry, AS CR   

                                                      Macromolecular beamlines at synchrotrons BESSY, XFEL

 Experimental techniques in protein crystallography

  Jiří Brynda, Institute of Organic Chemistry and Biochemistry, AS CR   

                                                      Home protein diffractometers , and diffraction beamlines in Hamburg    

  Jiří Brynda, Petr Pachl, Institute of Organic Chemistry and Biochemistry, AS CR   

                                                 Practical examples. Indexation, calculation of intensities measured by area detectors, structure determination.                                                           

 Time resolved experiments

   Jindřich Hašek, Institut of Macromolecular Chemistry, AS CR                              

                                                      Structure changes during chemical and biochemical reactions          

  Small-angle scattering

  Jan Ilavský, Argonne National Laboratory, USA                                                                          

                                                      Introduction SAXS techniques, SAXS – basic notions, instrumentation       

              Evaluation of small-angle scattering experiments, ultra-Small-Angle X-ray Scattering, Irena program package  

  Václav Holý, Faculty of Mathematics and Physics, Charles University in Prague                                                                        

                                                      GISAXS - theory, experimental equipment, some results

Scattering of thermal neutrons

 Jiří Kulda, Institute Laue-Langevin, Grenoble, France                                                                            

                                                      Diffraction of thermal neutrons diffraction. Neutron scattering -:  techniques and use in structure analysis

 Pavel Strunz, Jaromír Šaroun, Vasyl Ryukhtin, Institute of Nuclear Physics, Řež near Prague

               Role of SANS in a design of new materials, Evaluation of SANS measurements                                                                                           

Applications of protein crystallography

 Bohdan Schneider, Institute of Biotechnology, Praha, AS CR                                                       

                                                       Bioinformatic analysis of crystal protein-DNA complexes                               

 Ivana Kutá Smatanová, University of West Bohemia, České Budějovice                                                                                            

                                                       Crystallographic studies of selected halogenalkandehalogenases            

 Michal Kutý, University of West Bohemia, České Budějovice                                                     

                                                       Structura and function of photosynthetic proteins                                             

Powder diffraction

Radomír Kužel, Faculty of Mathematics and Physics, Charles University in Prague

                                                       Powder diffraction - instrumentation, information, evaluation

Ľubomír Smrčok, Institute of Inorganic Chemistry, Slovak Academy of Sciences

                                                       Structure refinement from powders by minimization of total energy        

STUDENT MICROSYMPOSIUM

Molecular structure

1.    J. Stránský:    Structure features and regulation markers of the plant nuclease TBN1

2.    T. Holubeva:  Isolation and crystallization of PSII from higher plants with different content of detergent

3.    I. Iermak: Crystallization of the members of multistep signaling system from Arabidopsis thaliana

4.    E. Tutubalina: Using Ionic Liquids in Protein Crystallization

5.    J. Pisáčková:   Towards Structures Of Antibodies, Receptors and Transcription Factors

6.    O. Degtjiarik:  Structural characterization of the members of the multistep signaling system from A. thaliana

7.    K. Tratsiak:    Crystal structure of haloalkane dehalogenase DpcA from psychrophilic Psychrobacter cryohalolentis K5

8.    G. Demo: Crystallization and X-ray analysis of beta-D-mannosidase from Aspergillus Niger

Materials science

1.    V. Valeš:  Nanočástice Fe₂O₃/TiO₂ - komplexní strukturní studium

2.    T. Brunátová: Studium struktury a teplotní stability titanátových nanotrubek

3.    J. Růžička: Studium manganem dopovaných topologických izolátorů Bi₂Se₃ a Bi₂Te₃ pomocí rtg absorpční spektroskopie

4.    J. Rozbořil: Strukturní vlastnosti epitaxních Ge mikrokrystalů na Si

5.    J. Endres:  Kinetické Monte Carlo simulace růstu multivrstev kvantových teček Ge v amorfní matrici

6.    K. Richterová: Výzkum struktury Heuslerových sloučenin

7.    J. Čapek:  Analýza stavu zbytkové napjatosti texturovaných materiálů

8.    M. Kučeráková: Studium textur slitin zirkonia neutronovou a rentgenovou difrakcí

9.    R. Uhrecký: Príprava a štruktúrne vlastnosti manganitých komplexov s kyselinou pyridin-2,6-dikarboxylovou