Crystallization Course - Programme


Topics

 

Speakers

Crystallization

Phase diagram

Properties of solution

Nucleation

Crystal growth

Applied crystallization

Solving of structures

Diffraction physics

Synchrotron radiation

Data collection

Phasing methods

Refinement

Rolf Hilgenfeld (Germany)
Thomas Klupsch (Germany)
Juan Manuel Garcia-Ruiz (Spain)
Jeroen Mesters (Germany)

Jiří Brynda (Czech Republic)
Jindřich Hašek (Czech Republic)
Ivana Kutá Smatanová (Czech Republic)
Bohdan Schneider (Czech Republic)


Daily Schedule

Friday, October 5

16:00 - 22:00 Registration

Saturday, October 6

8:00 Registration

9:00 Breakfast

10:00 Introduction and welcome 

10:15 Organization remarks

Lectures

10:30 Overview + Introduction to crystallography

11:00 Coffeee

11:20 - 12:50 Principles of crystallization: Solution properties, phase diagram and nucleation

13:00 Lunch

Practices

14:30 Crystallization trials

18:30 Dinner

Social program

20:00 Visit of castle + concert in Monastery

 

Sunday, October 7

8:15 Breakfast

Lectures

9:00 Protein expression and purification 

10:00 Principles of crystallization: Crystal growth mechanisms and processes

11:00 Coffee

11:20 - 12:45 Crystallization techniques (Batch, thermal and vapour diffusion)

13:00 Lunch

Practices

14:30 Automates for protein crystallization, robotics

18:30 Dinner

Social program

20:00 Movie

 

 

Monday, October 8

8:15 Breakfast

Lectures

9:00 Growth kinetics

10:00 Advanced crystallization techniques (counter-diffusion methods)

11:00 Coffee

11:20 - 12:00 Protein databases

12:30 Lunch

Social program

13:30 Trip to Cesky Krumlov

19:00 Official dinner

 

Tuesday, October 9

8:15 Breakfast

Lectures

9:00 Diffraction theory

10:00 Data collection

11:00 Coffee

11:20 - 12:20 Using of electrons and neutrons for structure determination as an alternative to X-ray

13:00 Lunch

Practices

14:00 Check of crystallization set-ups, crystallization techniques, freezing  of crystals

18:30 Dinner

20:00 Free time

 

Wednesday, October 10   PRAGUE

7:30 Breakfast

8:30 Departure from NH to Prague

11:30 Lunch

Practices

12:30 How to use a diffractometer for collection of diffraction data 

16:30 Return to NH

19:30 Dinner

20:00 Free time

 

Thursday, October 11

8:15 Breakfast

Lectures

9:00 Phasing methods

10:00 Refinement

11:00 Coffee

11:20 The use of synchrotron radiation in PX

12:30 Lunch

Practices

14:00 Solving of structures using PX software

18:30 Dinner

Social program

20:00 Resume and small party in the castle

 

Friday, October 12

8:15 Breakfast

8:00 - 10:00 Departure