The Protein Production core facility (CF) at the Centre of Molecular Structure (CMS) offers comprehensive protein production services, covering every step from DNA to the purified protein. These include gene cloning into expression vectors, site-directed mutagenesis, and heterologous expression in Escherichia coli expression systems, followed by protein purification.
Our cloning services include both traditional cloning using restriction enzymes and restriction‑free (RF) methodologies. You can provide us with your templates, or we can order them from external companies. Subsequently, we can deliver and test a number of our plasmids. Furthermore, we perform small-scale expression and solubility tests using various E. coli strains under different conditions. Finally, we offer large-scale production and purification of target proteins.
In protein purification, we employ a range of affinity techniques such as Strep-Tactin XT, and immobilized metal chelate affinity chromatography (IMAC), both on FPLC or in gravity flow setups. Chromatography techniques include ion-exchange chromatography and size exclusion chromatography using Superdex columns (75 or 200, 10/300 increase, or HiLoad 16/600). You can request customization to our standardized protocols or provide your established protocols or implementation.
Moreover, we are expanding our services by introducing eukaryotic production during this year. We will provide protein production using human embryonic kidney cells (HEK) and Sf9 insect cells as an alternative to prokaryotic expression in E. coli.
The Biocev Protein Production core facility is a part of CMS operated by the Institute of Biotechnology, Czech Academy of Sciences. The Centre of Molecular Structure is supported by: Czech Infrastructure for Integrative Structural Biology (CIISB), Instruct-CZ Centre of Instruct-ERIC EU consortium, funded by MEYS CR infrastructure project LM2023042). European Regional Development Fund-Project „UP CIISB” (No. CZ.02.1.01/0.0/0.0/18_046/0015974).