Biophysical techniques at Centre of molecular structure of BIOCEV

Tatsiana Charnavets, Bohdan Schneider, Jan Dohnálek

Institute of Biotechnology, BIOCEV, Prùmyslova 595, Vestec, 25250, Czech Republic

The biophysical research facility is a part of the Centre of molecular structure of Institute of Biotechnology. Facility is a shared resource that provides an access to instruments, technologies, expert consultation and training to researchers.

For the determination of size, molecular mass, structure and stability of biomolecules, study of conformational changes and thermodynamics of temperature transitions are currently available: mass photometry (Two MP mass photometer) circular dichroism spectroscopy (Chirascan Plus CD spectrometer), spectrophotometry (Specord 50 Plus UV/Vis spectrophotometer), Fourier-transform infrared spectrometry (Vertex 70v spectrometer), fluorescence spectrometry (photoluminescence spectrometer FLS1000), differential scanning fluorescence (Prometheus NT.48), multiangle dynamic light scattering (Zetasizer Ultra), microplate reader (Tecan), differential scanning calorimetry (Microcal VP-DSC). Isothermal titration calorimetry (Microcal iTC200 and PEAQ-ITC), microscale thermophoresis (Monolith NT.115 and NT.LabelFree), surface plasmon resonance (ProteOn XPR36) and bio-layer Interferometry (OCTET R8) techniques are available for the characterization of biomolecular interactions.

Facility is a member of Instruct-ERIC, Czech Infrastructure for Integrative Structural Biology (CIISB) and Molecular-Scale Biophysics Research Infrastructure (MOSBRI).

All relevant information is on the web pages: https://www.ibt.cas.cz/cs/servisni-pracoviste/centrum-molekularni-struktury/, https://www.ciisb.org/open-access/core-facilities.

The Centre of Molecular Structure is supported by: MEYS CR (LM2023042); project UP CIISB (CZ.02.1.01/0.0/0.0/18_046/0015974), CIISB4HEALTH (CZ.02.1.01/0.0/0.0/16_013/0001776); ELIBIO (CZ.02.1.01/0.0/0.0/15_003/0000447), and MOSBRI (No. 101004806).