Pushing the Boundaries in Biomolecular Interaction Analysis with GCI and waveRAPID
M. Dudák1, G. Buijs2
1ANAMET s.r.o., Kovákù 3210/26, 150 00, Praha 5, Czech Republic
2 Creoptix AG, Zugerstrasse 76, CH–8820 Wädenswil, Switzerland
dudak@anamet.cz
The WAVEsystem with the Grating-coupled interferometry (GCI) is the novel surface-based, label-free biosensing technology to measure binding kinetics and affinity, with superior resolution in signal and time compared to SPR. The WAVEsystem allows researchers to quickly and accurately measure label-free binding kinetic rates, determine affinity constants, and monitor the concentrations of even low abundance interacting analytes in crude samples such as biofluids. Paired with no-clog WAVEchips®, a wide range of molecules can be immobilized using various chemistries or capturing techniques, while remaining compatible with crude samples or complex matrices. The Creoptix® WAVEsystem combines the enhanced sensitivity of GCI technology with the high throughput method waveRAPID® to reduce screening time and complexity.
GCI is our cutting-edge biophysical characterization method commercially available since 2015 in the WAVE family of laboratory devices. Its evanescent field penetrates less deep into the sample and extends the light-to-sample interaction length for improved signal-to-noise ratios (<0.01 pg/mm2). Standard immobilization techniques such as amine-coupling, Ni-NTA capture and streptavidin-biotin capture are available for the Creoptix WAVEsystem, as well as alternative lipidic interactions or Protein A/G capture. It is suitable for Fragments, Small Molecules, Peptides, Proteins, Viruses, Cell Culture Supernatants, Serums, Cell lysates etc.
The above statements will be supported by application data.
