Bacterial Antibiotic REsistance ABC ATPases F (ARE ABC-F) are cytosolic proteins conferring resistance to 50S ribosomal subunit binding antibiotics via target protection mechanism. Expression of all previously characterised ARE ABC-F genes that confer resistance to peptidyl transferase centre targeting antibiotics lincosamides, streptogramins A and pleuromutilins (LSAP) is regulated by ribosome-mediated attenuation in response to these antibiotics, however the molecular mechanism is lacking. Considering that antibiotic-driven attenuation generally requires sequence-dependent translational arrest, we would expect the same also for LSAP-driven attenuation. The aim of our research is to shed light on the mechanism of LSAP-induced translation inhibition using ribosome profiling techniques (toeprinting and high-throughput inverse toeprinting) and single particle cryo-EM and based on these data, to clarify the expression regulation of ARE ABC-Fs in pathogens and antibiotic producers.