Core Facility of High Field NMR Spectroscopy provides access to NMR spectrometers in the range of proton frequencies from 500 MHz to 950 MHz. The equipment is suited mainly to the studies of structure, dynamics, and interactions of biomolecules, i.e., proteins, nucleic acids, and carbohydrates. However, the instrumentation is flexible enough to cover also various research needs in material science, organic and inorganic chemistry, biochemistry, biology, and biophysics.
Available is the following instrumentation:
} NMR Spectrometer Bruker AVANCE NEO 500 MHz – available with a 5 mm nitrogen-cooled multinuclear cryoprobe (Prodigy), room temperature 5 mm dual broad-band probe (1H, 15N - 19F), triple-resonance (1H, 13C, 15N) 5 mm probe, 10 mm dual (1H, 13C) probe, and 4.0 mm solid-state dual CP/MAS probe.
} NMR Spectrometer Bruker AVANCE NEO 600 MHz equipped with a quadruple-resonance (1H/19F, 13C, 15N, 31P) cryoprobe with -40 to 150⁰C temperature range.
} NMR Spectrometer Bruker AVANCE NEO 700 MHz for biomolecular applications, equipped with a triple-resonance (1H/19F, 13C, 15N) cryoprobe with -40 to 150⁰C temperature range and a triple-resonance (1H, 13C, 15N) cryoprobe optimized for detection of 13C, -40 to 80⁰C temperature range.
} NMR Spectrometer Bruker AVANCE NEO 700 MHz for multinuclear applications, equipped with a 5 mm dual broad-band probe, 5 mm dual inverse broad-band probe, 1.7 mm triple resonance (1H, 13C, 15N) probe, 5 mm dual broad-band probe for diffusion measurements, 3.2 mm high-resolution solid-state triple-resonance (1H, 13C, 15N) MAS probe, and 4.0 mm solid-state dual CP/MAS probe.
} NMR Spectrometer Bruker AVANCE NEO 850 MHz equipped with a triple-resonance (1H/19F, 13C, 15N) cryoprobe with 0 to 135⁰C temperature range and a triple-resonance (1H, 13C, 15N) cryoprobe optimized for detection of 13C, -40 to 150⁰C temperature range.
} NMR Spectrometer Bruker AVANCE NEO 950 MHz equipped with a triple-resonance (1H/19F, 13C, 15N) cryoprobe, -40 to 150⁰C temperature range.
} X-Band (9,75 GHz) EPR spectrometer Bruker EMX nano.
} Bioreactor system for NMR studies of proteins/nucleic acids in living mammalian cells
Services provided:
} Measuring of NMR spectra at magnetic fields from 11.75 T to 22.32 T (corresponding to proton frequencies from 500 MHz to 950 MHz) for compounds identification and quality control.
} Consultations concerning the choice and setup of multidimensional NMR experiments, data processing and spectra evaluation according to the users’ needs.
} Structure analysis of organic compounds by NMR spectroscopy.
} Studies of structure and dynamics of proteins and nucleic acids by NMR including measurement and processing of multidimensional spectra of proteins with up to 5 dimensions using non-linear sampling.
} Mapping of minor conformations (invisible states) by relaxation dispersion.
} Studies of interactions using STD (Saturation Transfer Difference) spectroscopy.
} In vivo conformational studies of biomolecules in cell.
} Investigation of material structure by solid state NMR spectroscopy of 13C, 31P, 29Si, and 27Al.
The centre is available to academic user from around the world for free or a fraction of the operation cost through CIISB, iNEXT-Discovery, instruct ERIC, and Alliance4Life grants. The project proposals can be submitted through the web pages of the respective grants.
https://www.ciisb.org/open-access/proposal-submission
https://inext-discovery.eu/submit-proposal/
https://alliance4life.ceitec.cz/core-facility-database-and-proposal-submission/
Industrial users can access the services of the Centre on commercial bases.