When protein stability matters

Piotr Wardega

NanoTemper Technologies spzoo

Prometheus characterizes thermal and chemical unfolding under native conditions using nanoDSF technology. Our technology is completely label-free; it precisely measures the intrinsic fluorescence of a protein while it is being subjected to either chemical, time-resolved or most commonly, thermal denaturation.

The fluorescence of tryptophan in a protein is strongly dependent on its close surroundings. By following changes in fluorescence various stability describing parameters can be assessed in a truly label-free manner. The dual-UV technology developed by NanoTemper allows for rapid signal detection, providing an unmatched scanning speed as well as data points density. This approach yields an ultra-high resolution unfolding curves which translate to very accurate detection of even minute unfolding signals, allowing for better evaluation and understanding of studied protein’s structure. Furthermore, since no fluorophores , for protein labeling, are required as in historical DSF, sample solutions are analyzed independent of buffer nature or compositions.

nanoDSF uses high quality glass capillaries to provide— impressive sample saving- only microliters are required, -wide range of concentrations which are quickly measured in solution, and hard-to-measure viscous samples are examined with no problem.

Experiments performed with Prometheus platform deliver T_m, T_onset and T_agg , C_m, ΔG and ΔΔG as well as time resolved parameters in isothermal conditions.

Our Prometheus allows for parallel measurement of protein aggregation propensity of tested samples, providing valuable information on their colloidal state.

All above make nanoDSF, and specifically Prometheus platform,  the method of choice for easy, fast and precise analysis of protein folding and stability.

On the practical note; Prometheus does not require maintenance, flushing, equilibration or any other between-experiments interventions, making it money saving and multi users- friendly, as samples solutions do not come in direct contact with the instrument.

To discuss your specific research interest we invite you to visit our company booth during the meeting.