The biophysical research facility as a part of the Centre of molecular structure of Institute of Biotechnology - member of Instruct-ERIC and Czech Infrastructure for Integrative Structural Biology, provides shared resources of instruments and technologies for the determination of size, structure and stability of biomolecules, study of conformational changes and thermodynamics of temperature transitions and characterization of biomolecular interactions.
Following instruments and technologies are currently available: circular dichroism spectroscopy (Chirascan Plus CD spectrometer), spectrophotometry (Specord 50 Plus UV/Vis spectrophotometer), Fourier-transform infrared spectrometry (Vertex 70v spectrometer), fluorescence spectrometry (photoluminescence spectrometer FLS1000), differential scanning fluorescence (Prometheus NT.48), multiangle dynamic light scattering (Zetasizer Ultra) and differential scanning calorimetry (Microcal VP-DSC), isothermal titration calorimetry (Microcal iTC200), microscale thermophoresis (Monolith NT.115 and NT.LabelFree) and surface plasmon resonance (ProteOn XPR36).
This work was supported by the ERDF (BIOCEV: CZ.1.05/1.1.00/02.0109), MEYS CR (CIISB: LM2018127, CIISB4HEALTH).