When Affinity Matters

Pawel Kania

NanoTemper Technologies CEE

When affinity matters

At NanoTemper Technologies, we are passionate about achieving excellence in everything we do. This has made us the partner of choice for thousands of researchers worldwide in pharmaceutical and biotechnology industries as well as in academic research institutes.

The foundation of NanoTemper Technologies is microscale thermophoresis (MST), a powerful technique to quantify biomolecular interactions. MST is a biophysical technique that measures the strength of the interaction between two molecules by detecting variations in fluorescence signal as a result of an IR-laser induced temperature change. The range of the variation in the fluorescence signal correlates with the binding of a ligand to the fluorescent target. MST signal strongly depends on a variety of molecular properties such as size, charge, hydration shell or conformation. Thus, this technique is highly sensitive to virtually any change in molecular properties, allowing for a precise quantification of molecular events independent of the size or nature of the investigated specimen.  Along, MST with its unique capabilities enables researchers to work with unpurified proteins, directly in their close to native conditions, such as cell or tissue lysate and membrane fraction as well.

When screening matters

Our newest platform is a result of a constant evolution of our core technology to respond to expectations coming from NanoTemper Technologies customers- Dianthus NT.23.  Dianthus NT.23 brings a breakthrough in novel biophysical tactic to high throughput screening approach and removes the complexity of binding interaction measurements for drug discovery, while providing a standardization and automation friendly 384-wellls plate format.

Dianthus NT.23 allows for:

ü Working with even the most challenging targets, previously inaccessible, regardless whether they are nucleic acids, multimeric proteins without enzymatic function or any other

ü Measuring affinities with the highest sensitivity spanning from pico- to millimolar range, covering all needs in the most projects

ü Ensuring minimal sample consumption, saving on expensive targets as well as allowing for very small test compounds use

ü Free use of literally any buffer of choice, even cell lysates or any other bioliquid, enabling the best possible scenario for studied drug discovery target corresponding to its most native state

ü Work in free solution, where no immobilization required, studied molecules are available for interactions in all dimensions and no extra time to work on expensive immobilizing chemistries is necessary

ü Test more than 8.000 compounds in duplicate in 24 hours and collect vast amount of meaningful data for further studies

To discuss your specific research interest we invite you to visit our company booth during the meeting.