Domain Integrity Verification of Alternative Splicing

Domain Integrity Verification of Alternative Splicing - the DIVAS server

Tamas Horvath¹, Lajos Kalmar¹ , Nigel Brown² and Hedi Hegyi^2,*

¹ Institute of Enzymology; Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary

² CEITEC - Central European Institute of Technology, Masaryk University, CZ-62500 Brno, Czech Republic
hegyi@ceitec.muni.cz

We present a new method and a server to predict the viability of proteins with globular domains truncated by alternative splicing or chromosomal translocation. The prediction is based on residue-level calculations of exposed hydrophobicity of structural domains with minimum 60% sequence similarity to the query, combined with local disorder values and domain length statistics. The accuracy of the server varies between 75% (for TargetDB) and 88% (Swissprot with protein evidence).