Complementation of 3D structure of delta subunit of RNA polymerase from Bacillus subtilis with description of internal motions in terms of reduced spectral density mapping

P. Kadeřávek^1,2, C. Diehl³, V. Papoušková^1,2, H. Šanderová⁴, P. Padrta^1,2, L. Žídek^1,2, L. Krásný⁴, V. Sklenář^1,2, M. Akke³

¹National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Kotlářská 2,

CZ 611 37 Brno, Czech Republic

²CEITEC, Masaryk University, Žerotínovo náměstí 617/9, CZ 601 77 Brno, Czech Republic

³Center for Molecular Protein Science, Biophysical Chemistry, Lund University, Getingevägen 60,

SE 22100 Lund, Sweden

⁴Laboratory of Molecular Genetics of Bacteria and Department of Bacteriology, Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083 , CZ 142 20 Prague, Czech Republic

kada@ncbr.chemi.muni.cz

RNA polymerases of Gram positive and Gram negative bacteria differ. The subunit composition in Gram positive bacteria includes two additional subunits. One of them is denoted δ-subunit. Structure of δ-subunit of Bacillus subtilis has been solved recently using methods of solution NMR [1]. Here, we extend the information about the structure by the basic characterization of its dynamics studied at two temperatures. The standard relaxation experiments (R₁, R₂, ssNOE) were performed and data were analyzed using reduced spectral density mapping as the most straightforward approach.

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