Structural study of LEDGF cellular binding partners
K. Prochazkova 1, M. Horejsi 2, K. Cermakova 1, P. Rezacova 1,2.
1Institute of Organic Chemistry and BiochemistryAS CR, Flemingovo nam. 2, 166 10, Prague 6, Czech Republic
2Insitute of Molecular Genetics AS CR, Flemingovo nam. 2, 166 10, Prague 6, Czech Republic
katerina.prochazkova@uochb.cas.cz
rezacova@img.cas.cz
Lens epithelium-derived growth factor p75
While the role of LEDGF/p75 in HIV integration is well characterized, very little is known about its physiological function. As a transcriptional co-activator, LEDGF/p75 is implicated not only in HIV replication, but also in human cancer and autoimmunity. The LEDGF/p75 was shown to interact through its IBD with several cellular proteins and recent evidence implies that LEDGF/p75 is a general adaptor protein tethering various factors to chromatin [2].
In this work, we set to prepare two
LEDGF/p75 physiological binding partners JPO2 [2] and pogo transposable element
The aim of our study is to obtain structural information on the LEDGF/p75 interaction with its physiological binding partners JPO2 and pogZ, respectively. Such structural information is essential for understanding the LEDGF/p75 biological role and might help in design of inhibitors selectively blocking interaction with HIV integrase while not interfering with the LEDGF/p75 biological function.
References
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Maertens, P. Cherepanov, A. Engelman, J Cell Sci, 119, (2006), 2563.
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Acknowledgements
This work was supported by grant
from the FP7 framework of the European Union