Orientational restraints in NMR structure

L. Žídek, P. Padrta, J. Chmelík

Masaryk University, Faculty of Science, NCBR, Kotlářská 2, 61137 Brno, Czech Republic

The lecture will provide a general introduction to the restraints obtained in partially aligned samples of biomacromolecules. Methods of alignment will be mentioned and observed interactions of different physical origins will be briefly discussed. Methods of validation of the obtained data prior to structure calculation will be presented.