NMR study of protein CD69


M. Nálezková, D. Kavan, L. Gorčík, J. Chmelík, L. Žídek, K. Bezouška, V. Sklenář


1National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Czech Republic

2Institute of Microbiology, Academy of Sciences of the Czech Rep., Vídenská 1083, Praha 2



Keywords: NMR, CD69, glycoproteins


Protein CD69 is transmembrane disulphide-linked glycoprotein of natural killer cells, which are important in the immune response to cell-surface displayed antigens. C13 and N15 labeled sample was prepared for NMR experiments. Resonance frequencies of  74% backbone atoms and 39% side chains atoms have been assigned. Titration experiments with various mono- and oligosaccharides have been performed. N15 relaxation data have been measured and used to calculate the rotational diffusion coefficient. The results showed that the studied protein was present in a form of a dimer and that it exhibited specific binding affinity towards several ligands.