M. Nálezková, D. Kavan, L. Gorčík, J. Chmelík, L. Žídek, K. Bezouška, V. Sklenář
1National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Czech Republic
2Institute of Microbiology, Academy of Sciences of the Czech Rep., Vídenská 1083, Praha 2
Keywords: NMR, CD69, glycoproteins
Protein CD69 is transmembrane disulphide-linked glycoprotein of natural killer cells, which are important in the immune response to cell-surface displayed antigens. C13 and N15 labeled sample was prepared for NMR experiments. Resonance frequencies of 74% backbone atoms and 39% side chains atoms have been assigned. Titration experiments with various mono- and oligosaccharides have been performed. N15 relaxation data have been measured and used to calculate the rotational diffusion coefficient. The results showed that the studied protein was present in a form of a dimer and that it exhibited specific binding affinity towards several ligands.