Overexpression, purification and crystallization attempts of the recombinant R subunit EcoAI
M. Lapkouski and E. Jindrova
Institute of Physical Biology,
Email lapkouski@greentech.cz
Type I DNA restriction enzymes are large
molecular machines comprising DNA methyltransferase, ATPase, DNA translocase
and endonuclease activities, essential for the antivirus defence in bacterial
cells. The ATPase, DNA translocase and endonuclease activities are specified by
the restriction (R) subunit of the enzyme. Our work is focused mainly on
revealing structural features of the R subunit of the endonuclease EcoAI
from Escherichia coli. Having optimized purification methods,
crystallization trials were performed as a step for farther structural
characterization of the enzyme.
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This research was supported by the Ministry
of Education, Youth and Sports of the Czech Republic
(MSM6007665808, LC06010) and by the
research concept AVOZ60870520)