Overexpression, purification and crystallization attempts of the recombinant R subunit EcoAI
M. Lapkouski and E. Jindrova
Institute of Physical Biology,
Type I DNA restriction enzymes are large molecular machines comprising DNA methyltransferase, ATPase, DNA translocase and endonuclease activities, essential for the antivirus defence in bacterial cells. The ATPase, DNA translocase and endonuclease activities are specified by the restriction (R) subunit of the enzyme. Our work is focused mainly on revealing structural features of the R subunit of the endonuclease EcoAI from Escherichia coli. Having optimized purification methods, crystallization trials were performed as a step for farther structural characterization of the enzyme.
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This research was supported by the Ministry of Education, Youth and Sports of the Czech Republic
(MSM6007665808, LC06010) and by the
research concept AVOZ60870520)