Conference Topics (Lectures)
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Protein preparation for biophysical studies (solubility, phase diagram, Hofmeister series, purity, activity)
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Advanced electrophoresis techniques (native electrophoresis, zige, 2d-electrophoresis)
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Protein/protein and protein/nucleic acid complex preparation for biophysical studies (AS-precipitation, liquid chromatography, Kd)
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Protein engineering (SER, intelligent mutations, "crosslinking", linkers "GGSGG", etc)
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Dynamic and static light scattering (analysis of biological unit, complex formation)
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MS (deuterium exchange MS)
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Single molecule techniques (labelling)
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ITC DSC (Kd, buffer screening)
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Thermal shift assay Thermofluor/DSF
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NMR (1H-NMR, STD-NMR)
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SAXS and XFEL
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X-ray & Neutron diffraction
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Microscale thermophoresis
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Ultrafast laser spectroscopy as a tool for studies of protein function
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CD
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Electron Microscopy
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Computational methods
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WWW Resources
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AUC = analytical ultracentrifugation
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SPR
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Integrative structural approach to study tRNA : protein complexes (including DLS, SAXS, SRCD, MST, DSF, AUC and XRD)
Lecture
context (approx. 15 slides):
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Introduction and background of the technique used for research
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Little theory (what you can do with this techniques, how you can
study your proteins)
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Pictures
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2-3
examples + references
Plenary lectures
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Alexandra Zahradníková, Bratislava,
Slovakia: Role of the N-terminal region in ryanodine receptor channel
activation
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Beata Vertessy, Hungary:
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