Conference Topics (Lectures)

  • Protein preparation for biophysical studies (solubility, phase diagram, Hofmeister series, purity, activity) 

  • Advanced electrophoresis techniques (native electrophoresis, zige, 2d-electrophoresis) 

  • Protein/protein and protein/nucleic acid complex preparation for biophysical studies (AS-precipitation, liquid chromatography, Kd)

  • Protein engineering (SER, intelligent mutations, "crosslinking", linkers "GGSGG", etc) 

  • Dynamic and static light scattering (analysis of biological unit, complex formation)  

  • MS (deuterium exchange MS)

  • Single molecule techniques (labelling)

  • ITC DSC (Kd, buffer screening)

  • Thermal shift assay Thermofluor/DSF 

  • NMR (1H-NMR, STD-NMR)

  • SAXS and XFEL

  • X-ray & Neutron diffraction

  • Microscale thermophoresis

  • Ultrafast laser spectroscopy as a tool for studies of protein function

  • CD

  • Electron Microscopy 

  • Computational methods

  • WWW Resources

  • AUC = analytical ultracentrifugation

  • SPR

  • Integrative structural approach to study tRNA : protein complexes (including DLS, SAXS, SRCD, MST, DSF, AUC and XRD)

Lecture context (approx. 15 slides):

  1.        Introduction and background of the technique used for research

  2.        Little theory (what you can do with this techniques, how you can study your proteins)

  3.           Pictures

  4.               2-3 examples + references

Plenary lectures

  1. Alexandra Zahradníková, Bratislava, Slovakia: Role of the N-terminal region in ryanodine receptor channel activation

  2. Beata Vertessy, Hungary: