The immune-electron microscopic localization of molecules of interest by monoclonal and polyclonal antibodies in cells and tissues has become an indispensable routine technique in numerous fields of biological and biomedical research. It is based on a specific bond between antigen and specific antibodies conjugated with a high-density gold nanoparticle tag, which can be visualized usually by transmission electron microscope (TEM). The aim of my contribution is to give an overview of new methods of immuno-localization aimed at simultaneous multiple detection of molecules of interest, which are based on using low voltage electron microscopy and correlative light and electron microscopy.