2 Institute of Biotechnology, Czech Academy of Sciences, Průmyslová 595, 252 50 Vestec, Czechia
Interferon gamma (IFNg) is an important molecule for the immune system of vertebrates. The Protein Data Bank contains structures of IFNg from numerous species. We crystallized and collected the diffraction images of IFNg from the olive flounder - Paralichthys olivaceus. The raw diffraction images of native and Selenoure soak of PoIFNg have been deposited in the Zenodo public repository (native – https://doi.org/10.5281/zenodo.3369717, Selenourea soak – https://doi.org/10.5281/zenodo.19218271). Initially, we estimated the diffraction limit at 2.3 Å. We solved the phase problem using the single-wavelength anomalous dispersion method and deposited the structure to the PDB under the accession code 6F1E [1].
The manuscript and data is great for teaching multiple stages of structure determination including the practical approaches. First of all, the Materials and Methods contains a protocol for production of crystals for experimental phasing. In addition, the diffraction images are available for reproduction of data reduction and further analysis. The diffraction anisotropy can be treated using modern approaches and result in better data. The structure of PoIFNg is different from the predicted models or from models of homologous proteins known by the year of its publication (2018). The experimental phases may serve for both phase problem solution and final model validation. The dataset is already used in tutorial for teaching standard paired refinement. Even paired refinement considering diffraction anisotropy can be applied, although this process has not been implemented to any computational crystallographic pipeline, yet.