School on 3D electron diffraction/MicroED techniques and how to apply
them for structure determination of nano/microcrystals too small for
X-ray diffraction. The topics will cover inorganic compounds,
pharmaceutics and proteins. It includes both lectures and practical
sessions.
Topics:
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The development of various 3D electron diffraction/MicroED
techniques – a historical perspective
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Introduction to transmission electron microscopy and electron
diffraction
-
3D
electron diffraction/MicroED (SAED, PED, EDT) and space group
determination
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Sample preparation for both materials and life sciences
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3D ED/MicroED Data collection
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Radiation damage
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3D ED Data Processing and data merging
including practical demo labs
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Methods for phasing diffraction data – small molecules and
macromolecules
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Practical demo labs for structure solution and refinement with
3D ED/MicroED data – two groups
for
small molecules and macromolecules, respectively
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Dynamical refinement
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New developments in 3D electron crystallography
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