Theory and practice of crystallization
- Chemists view of the crystallization process
- Physical methods in protein crystallization
- Computational prediction of crystallization
- Scoring methods for evaluation of screens efficacy
Sample preparation and handling in advanced techniques
- classical crystallography
- small angle scattering (contrast in SAX)
- time-resolved crystallography
(biochemical processes on ms scale)
- femtosecond crystallography (XFEL)
- solid state NMR
- high resolution electron microscopy (HREM)
- electron diffraction
- neutron diffraction
Crystals for “high resolution studies”
- Diffraction quality of protein-ligand crystals (co-crystallization, soaking technigues leading to reliable identification of drug interactions)
- Room temperature measurements and controlled
hydration of crystals
- Cryo-protection, cryo-cooling and measurement
techniques
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Membrane proteins
- Crystallization of largely hydrophobic bio-macromolecules
- Crystallization techniques for cell receptor complexes - protein-protein molecular recognition
studies
Structure diversity of bio-macromolecules
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Polymorphy of proteins in solid state
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Temperature induced phase transitions
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Role of glycosylation on protein crystallization and function
Crystallization of conformationaly unstable proteins and other crystallization resisting (challenging) bio-macromolecules
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Targeted fragmentation, mutation, and purification
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Post-translational modifications, fusion constructs and other special approaches
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Co-crystallization with molecular chaperons
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Special protein expression systems for preparation of crystallizable protein complexes
Automation techniques in
crystallization
Crystallization in industry
Crystallization in drug design
Crystallography in education
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