Over one third of adults in America are obese and thus are at risk on type 2 diabetes, cancer and cardiovascular diseases. Phospolipase-Acyltranferase (PLA/AT) are important enzymes that are crucial in the development of obesity. We have recently designed a class of reversible PLA/AT inhibitors showing micromolar inhibition that are promising candidate anti-cancer or anti-obese drugs. The crystal structure of PLA1/AT3 and PLA2/AT3 have been reported [1], but the binding pocket is very large and consequently the inhibitors may have multiple binding modes, Here, we describe the crystallization and preliminary structure analysis if PLA/AT soaked with various inhibitors. Soaking PLA/AT protein crystals with inhibitors remains challenging, as the designed inhibitors are poorly soluble. Our results demonstrate that it was possible to obtain inhibitor soaked non-damaged crystals for PLA/AT 2, by varying the concentrations of inhibitor and various solubilizing agents, cryo protectant and protein crystal buffer solution. The resulting crystals were collected and resulted in high-resolution datasets of 1.4 Ă… respectively. We will optimize these results in future co-crystallization experiments or for the improvement of soaking other PLA/AT family members (1, 3-5) with a wide variety of inhibitors.